Scientists are applying a cell culturing technique called 'hanging drop culture' to better understand sweat glands. Typical cell cultures are grown in dishes that allow them to multiply and thrive in a two dimensional format. The hanging drop technique allows a cell culture to organize itself in a 3-dimensional fashion. As such, this technique allows cell cultures to |
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more realistically mimic the natural organization of cells within tissues. After all, sweat glands are 3-dimensional physiological units. Not only are the 3D cultures more lifelike, they also respond and function like real sweat glands. A numbers of tests have shown that 3D cultures behave more like real sweat glands. For example, adding acetylcholine (a neurotransmitter) to the 3-d cultures results in the influx of calcium within the cells. This process is normally observed within sweat glands. Cells cultures organized in typical 2D cultures do not respond to acetylcholine in this manner. Scientists are hoping the use of 3D sweat cell cultures will help determine which bioactive substances will best regulate the sweating process within these cells. *A novel organotypic 3D sweat gland model with physiological functionality. PLoS One. 2017 Aug 10;12(8):e0182752
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